Multi-scale visualization of dynamic changes in poplar cell walls during alkali pretreatment.
Identifieur interne : 002127 ( Main/Exploration ); précédent : 002126; suivant : 002128Multi-scale visualization of dynamic changes in poplar cell walls during alkali pretreatment.
Auteurs : Zhe Ji [République populaire de Chine] ; Jianfeng Ma [République populaire de Chine] ; Feng Xu [République populaire de Chine]Source :
- Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [ 1435-8115 ] ; 2014.
Descripteurs français
- KwdFr :
- Analyse spectrale Raman (MeSH), Cellules végétales (composition chimique), Cellules végétales (effets des médicaments et des substances chimiques), Facteurs temps (MeSH), Hydroxyde de sodium (métabolisme), Lignine (analyse), Microscopie (MeSH), Paroi cellulaire (composition chimique), Paroi cellulaire (effets des médicaments et des substances chimiques), Populus (composition chimique), Populus (cytologie), Populus (effets des médicaments et des substances chimiques), Température (MeSH).
- MESH :
- analyse : Lignine.
- composition chimique : Cellules végétales, Paroi cellulaire, Populus.
- cytologie : Populus.
- effets des médicaments et des substances chimiques : Cellules végétales, Paroi cellulaire, Populus.
- métabolisme : Hydroxyde de sodium.
- Analyse spectrale Raman, Facteurs temps, Microscopie, Température.
English descriptors
- KwdEn :
- Cell Wall (chemistry), Cell Wall (drug effects), Lignin (analysis), Microscopy (MeSH), Plant Cells (chemistry), Plant Cells (drug effects), Populus (chemistry), Populus (cytology), Populus (drug effects), Sodium Hydroxide (metabolism), Spectrum Analysis, Raman (MeSH), Temperature (MeSH), Time Factors (MeSH).
- MESH :
- chemical , analysis : Lignin.
- chemistry : Cell Wall, Plant Cells, Populus.
- cytology : Populus.
- drug effects : Cell Wall, Plant Cells, Populus.
- chemical , metabolism : Sodium Hydroxide.
- Microscopy, Spectrum Analysis, Raman, Temperature, Time Factors.
Abstract
Alkali pretreatment is a promising pretreatment technology that can effectively deconstruct plant cell walls to enhance sugar release performance. In this study, multi-scale visualization of dynamic changes in poplar cell walls during sodium hydroxide pretreatment (2% w/v, 121°C) was carried out by light microscopy (LM), confocal Raman microscopy (CRM) and atomic force microscopy (AFM). LM observations indicated that swelling occurred primarily in the secondary wall (S) but alkali had little effect on the cell corner middle lamella (CCML). Correspondingly, there was a preferential delignification in the S at the beginning of pretreatment, while the level of delignification in CCML (~88%) was higher than that in the S (~83%) for the whole process revealed by Raman spectra. It also suggested that prolonging residence time to 180 min would not remove lignin completely but cause rapid loss of carbohydrates, which was further visualized by Raman spectroscopy images. Furthermore, AFM measurements illustrated that pretreatment with alkali exposed the embedded microfibrils from noncellulosic polymers clearly, enlarged the diameter of microfibrils, and decreased the surface porosity. These results suggested that there was a synergistic mechanism of lignocellulose deconstruction regarding cell wall swelling, main components dissolution, and microfibril morphological changes that occurred during alkali pretreatment.
DOI: 10.1017/S1431927614000063
PubMed: 24548595
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada</title><idno type="eISSN">1435-8115</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (chemistry)</term><term>Cell Wall (drug effects)</term><term>Lignin (analysis)</term><term>Microscopy (MeSH)</term><term>Plant Cells (chemistry)</term><term>Plant Cells (drug effects)</term><term>Populus (chemistry)</term><term>Populus (cytology)</term><term>Populus (drug effects)</term><term>Sodium Hydroxide (metabolism)</term><term>Spectrum Analysis, Raman (MeSH)</term><term>Temperature (MeSH)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse spectrale Raman (MeSH)</term><term>Cellules végétales (composition chimique)</term><term>Cellules végétales (effets des médicaments et des substances chimiques)</term><term>Facteurs temps (MeSH)</term><term>Hydroxyde de sodium (métabolisme)</term><term>Lignine (analyse)</term><term>Microscopie (MeSH)</term><term>Paroi cellulaire (composition chimique)</term><term>Paroi cellulaire (effets des médicaments et des substances chimiques)</term><term>Populus (composition chimique)</term><term>Populus (cytologie)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Plant Cells</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellules végétales</term><term>Paroi cellulaire</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Wall</term><term>Plant Cells</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Cellules végétales</term><term>Paroi cellulaire</term><term>Populus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Sodium Hydroxide</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Hydroxyde de sodium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microscopy</term><term>Spectrum Analysis, Raman</term><term>Temperature</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse spectrale Raman</term><term>Facteurs temps</term><term>Microscopie</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Alkali pretreatment is a promising pretreatment technology that can effectively deconstruct plant cell walls to enhance sugar release performance. In this study, multi-scale visualization of dynamic changes in poplar cell walls during sodium hydroxide pretreatment (2% w/v, 121°C) was carried out by light microscopy (LM), confocal Raman microscopy (CRM) and atomic force microscopy (AFM). LM observations indicated that swelling occurred primarily in the secondary wall (S) but alkali had little effect on the cell corner middle lamella (CCML). Correspondingly, there was a preferential delignification in the S at the beginning of pretreatment, while the level of delignification in CCML (~88%) was higher than that in the S (~83%) for the whole process revealed by Raman spectra. It also suggested that prolonging residence time to 180 min would not remove lignin completely but cause rapid loss of carbohydrates, which was further visualized by Raman spectroscopy images. Furthermore, AFM measurements illustrated that pretreatment with alkali exposed the embedded microfibrils from noncellulosic polymers clearly, enlarged the diameter of microfibrils, and decreased the surface porosity. These results suggested that there was a synergistic mechanism of lignocellulose deconstruction regarding cell wall swelling, main components dissolution, and microfibril morphological changes that occurred during alkali pretreatment. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24548595</PMID><DateCompleted><Year>2015</Year><Month>04</Month><Day>15</Day></DateCompleted><DateRevised><Year>2014</Year><Month>04</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1435-8115</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>2</Issue><PubDate><Year>2014</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada</Title><ISOAbbreviation>Microsc Microanal</ISOAbbreviation></Journal><ArticleTitle>Multi-scale visualization of dynamic changes in poplar cell walls during alkali pretreatment.</ArticleTitle><Pagination><MedlinePgn>566-76</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1017/S1431927614000063</ELocationID><Abstract><AbstractText>Alkali pretreatment is a promising pretreatment technology that can effectively deconstruct plant cell walls to enhance sugar release performance. In this study, multi-scale visualization of dynamic changes in poplar cell walls during sodium hydroxide pretreatment (2% w/v, 121°C) was carried out by light microscopy (LM), confocal Raman microscopy (CRM) and atomic force microscopy (AFM). LM observations indicated that swelling occurred primarily in the secondary wall (S) but alkali had little effect on the cell corner middle lamella (CCML). Correspondingly, there was a preferential delignification in the S at the beginning of pretreatment, while the level of delignification in CCML (~88%) was higher than that in the S (~83%) for the whole process revealed by Raman spectra. It also suggested that prolonging residence time to 180 min would not remove lignin completely but cause rapid loss of carbohydrates, which was further visualized by Raman spectroscopy images. Furthermore, AFM measurements illustrated that pretreatment with alkali exposed the embedded microfibrils from noncellulosic polymers clearly, enlarged the diameter of microfibrils, and decreased the surface porosity. These results suggested that there was a synergistic mechanism of lignocellulose deconstruction regarding cell wall swelling, main components dissolution, and microfibril morphological changes that occurred during alkali pretreatment. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ji</LastName><ForeName>Zhe</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Jianfeng</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Feng</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>02</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Microsc Microanal</MedlineTA><NlmUniqueID>9712707</NlmUniqueID><ISSNLinking>1431-9276</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>55X04QC32I</RegistryNumber><NameOfSubstance UI="D012972">Sodium Hydroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008853" MajorTopicYN="Y">Microscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012972" MajorTopicYN="N">Sodium Hydroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013059" MajorTopicYN="N">Spectrum Analysis, Raman</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24548595</ArticleId><ArticleId IdType="pii">S1431927614000063</ArticleId><ArticleId IdType="doi">10.1017/S1431927614000063</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ji, Zhe" sort="Ji, Zhe" uniqKey="Ji Z" first="Zhe" last="Ji">Zhe Ji</name></noRegion><name sortKey="Ma, Jianfeng" sort="Ma, Jianfeng" uniqKey="Ma J" first="Jianfeng" last="Ma">Jianfeng Ma</name><name sortKey="Xu, Feng" sort="Xu, Feng" uniqKey="Xu F" first="Feng" last="Xu">Feng Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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